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Glutathione vs SS-31

Longevity

Glutathione

Endogenous thiol tripeptide and the principal intracellular antioxidant, central to redox buffering research.

Longevity

SS-31

Mitochondria-targeting tetrapeptide with high affinity for cardiolipin on the inner mitochondrial membrane.

Side by side

DimensionGlutathioneSS-31
Molecular weight

A 2.1× difference in mass — these are not comparable on a milligram-for-milligram basis.

307.32 g/mol639.79 g/mol
Size classsmall molecule / short peptideshort peptide
CAS number70-18-8736992-21-5
Research arealongevitylongevity
Sequenceγ-Glu-Cys-GlyD-Arg-Dmt-Lys-Phe-NH2
Available sizes750mg, 1500mg10mg
Entry price$39$49
Cost per mg

Cost per milligram differs by more than 3×. Compare against the concentrations your protocol actually requires, not the headline vial price.

$0.05$4.90

Mechanism

Glutathione

Glutathione is a tripeptide with an unusual γ-peptide bond between glutamate and cysteine, which confers resistance to standard peptidase cleavage. Its free thiol group makes it the dominant intracellular redox buffer, and the GSH/GSSG ratio is a standard readout of cellular oxidative state. It is also an obligate cofactor for glutathione peroxidases and S-transferases.

SS-31

SS-31 is a cell-permeable tetrapeptide that concentrates in the inner mitochondrial membrane through interaction with cardiolipin, a phospholipid essential to cristae architecture and respiratory supercomplex assembly. Its alternating aromatic-cationic motif drives membrane targeting independent of mitochondrial membrane potential — a mechanistic distinction from earlier targeting strategies such as TPP+ conjugation.

Research applications

Glutathione

  • GSH/GSSG ratio measurement as an oxidative stress readout
  • Glutathione peroxidase and S-transferase activity assays
  • Xenobiotic conjugation and detoxification pathway studies
  • Ferroptosis research (GPX4 dependence on GSH)

SS-31

  • Cardiolipin binding and cristae morphology studies
  • Mitochondrial respiration and ATP synthesis assays
  • Reactive oxygen species and oxidative stress models
  • Ischemia-reperfusion injury research

Common questions

What is the difference between Glutathione and SS-31?

Glutathione is endogenous thiol tripeptide and the principal intracellular antioxidant, central to redox buffering research. SS-31 is mitochondria-targeting tetrapeptide with high affinity for cardiolipin on the inner mitochondrial membrane. They differ in molecular weight (307.32 vs 639.79 g/mol) and in the pathways they are studied against.

Which is more cost-effective per milligram, Glutathione or SS-31?

Glutathione is lower at approximately $0.05 per milligram at its best tier, against $4.90 for the other. Cost per milligram is only meaningful relative to the concentrations a given protocol requires, since these compounds are not used at comparable masses.

Can Glutathione and SS-31 be studied together?

Combination designs appear in the published literature for a number of these compounds. Whether it is appropriate for a given protocol depends entirely on the model and endpoint. We supply research compounds and do not provide protocol guidance — consult the primary literature for your specific model.

How should Glutathione and SS-31 be stored?

Store lyophilized at -20°C protected from light. The free thiol oxidizes readily — minimize air exposure and prepare solutions fresh. For SS-31: Store lyophilized powder at -20°C protected from light. Reconstituted solution stable at 2-8°C for up to 30 days.

All products are intended strictly for laboratory research use. Not for human or veterinary use. Not for diagnostic or therapeutic use. Not a drug, food, or cosmetic.

Comparison generated from NuVida Vital catalog data. Every specification is reconciled against the certificate of analysis for the shipped batch.